Figure 5.

Verification that Syk, PKC, NF-κB, and cPLA₂ were related with PLE attenuated inflammation in DNP-IgE/BSA-induced RBL-2H3 cells. (a) (A) Images of RBL-2H3 cells stained by toluidine blue (magnification, ×400), (B) the percent of degranulation (%) (n = 3), (C) the release rate of β-Hex (%), and (D) the concentration of TNF-α in cells cotreated with PLE and Syk inhibitor (n = 3 independent experiments). (b) The release rate of β-Hex and (c) production of TNF-α in cells cotreated with PLE and PKC, NF-κB, or cPLA₂ inhibitor (n = 3 independent experiments). ^##P < 0.01vs. the control group; ^∗P < 0.05 and ^∗∗P < 0.01vs. the model group.