Fig. 5. Microfluidic platform results for murine breast tumor.

Breast tumors from MMTV-PyMT mice (n = 6) were resected, minced, processed with the microfluidic platform, and analyzed by flow cytometry. a EpCAM+ epithelial cells were ~2-fold higher at both time points. b Endothelial cells were enhanced even more by the microfluidic platform, with five- and ten-fold more cells recovered after 15 and 60 min, respectively. c Leukocytes increased by three- and five-fold after 15 and 60 min, respectively. The interval format produced similar total cell numbers relative to the corresponding static time point, except for endothelial cells, which were slightly higher. d Population distributions obtained for each processing condition. Device processing enriched both endothelial cells and leukocytes at all but the 1 min time point. Data are presented as mean values ± SEM from at least three independent experiments. Circles indicate values for experimental replicates. For stacked plots, experimental replicates are indicated by circles at 15 min, squares at 30 min, and triangles at 60 min. Two-sided T test was used for statistical testing. Stars indicate p < 0.05 and double stars indicate p < 0.01 relative to the control at the same digestion time. p values for all comparisons are presented in the Source Data file.