Table 2.
Metabolites of interest in ARDS patients controlling for confounders
| Metabolite | No Exclusions | Excluded Head AIS<3 |
Excluded Chest AIS<3 |
Excluded Baseline Lung Disease |
||||
|---|---|---|---|---|---|---|---|---|
| Fold Change |
p-value | Fold Change |
p-value | Fold Change |
p-value | Fold Change |
p-value | |
| Metabolites of Interest that met criteria (fold change>1.5 & p<0.05) | ||||||||
| Creatine | 2.14 | 0.0006 | 1.92 | 0.0255 | 2.00 | 0.0291 | 2.20 | 0.0010 |
| Succinate | 1.93 | 0.0016 | 2.28 | 0.0026 | 1.79 | 0.0203 | 1.97 | 0.0036 |
| Fumarate | 1.67 | 0.0109 | 1.78 | 0.0231 | 1.65 | 0.0163 | 1.59 | 0.0263 |
| Dehydroascorbate | 2.72 | 0.0013 | 2.74 | 0.0497 | 2.63 | 0.0291 | 2.72 | 0.0022 |
| Metabolites of Interest that did not meet criteria (fold change<1.5 & p>0.05) | ||||||||
| O-Propanoyl-Carnitine | 1.57 | 0.0168 | 1.69 | 0.0044 | 2.30 | 0.0646 | 1.60 | 0.0307 |
| sn-Glycerol-3-Phosphate | 4.04 | 0.0031 | 3.36 | 0.0683 | 4.42 | 0.0116 | 4.00 | 0.0053 |
| Spermine | 2.68 | 0.0156 | 1.83 | 0.2000 | 2.22 | 0.1105 | 2.32 | 0.0274 |
| Spermidine | 2.34 | 0.0273 | 1.94 | 0.2066 | 2.24 | 0.1387 | 2.31 | 0.0383 |
| Xanthine | 1.80 | 0.0025 | 1.74 | 0.0198 | 1.56 | 0.0834 | 1.70 | 0.0071 |
| Taurocholate | 3.91 | 0.0361 | 5.11 | 0.0619 | 3.03 | 0.1194 | 3.03 | 0.0491 |
| O-Butanoyl Carnitine | 1.73 | 0.0361 | 1.78 | 0.0327 | 1.34 | 0.1194 | 1.61 | 0.0427 |
| S-Adenosyl-Homocysteine | 1.13 | 0.0688 | 1.11 | 0.1180 | 1.14 | 0.1547 | 1.15 | 0.0711 |
| Malate | 1.37 | 0.0456 | 1.46 | 0.1419 | 1.25 | 0.1337 | 1.31 | 0.0861 |
| Taurine | 1.32 | 0.0046 | 1.19 | 0.0520 | 1.34 | 0.0237 | 1.34 | 0.0071 |
| Aspartate | 1.14 | 0.1672 | 1.13 | 0.4797 | 1.13 | 0.4478 | 1.19 | 0.1465 |
| Glutamate | 1.00 | 0.7629 | 0.98 | 0.9197 | 0.91 | 0.7293 | 1.00 | 0.6201 |
| 2-Oxoglutarate | 1.18 | 0.0777 | 1.04 | 0.3343 | 1.13 | 0.1194 | 1.19 | 0.0508 |
| Prostaglandin G-2 | 2.15 | 0.0849 | 2.34 | 0.0434 | 2.18 | 0.0800 | 1.93 | 0.1506 |
| Metabolites of interest in the plasma (Range (Median)) tested for PMN Priming | ||||||||
| Metabolite | Healthy Plasma Concentration (μM) |
Trauma Plasma Concentration (μM) |
Concentration Range Tested (μM) |
Significant Priming? |
||||
| Succinate | 0-5 | 7.9-301.3 (29.8) | 1-1,000 | Yes (Fig 1) | ||||
| Creatine | 30-80* | Not measured | 100-1,000 | No | ||||
| Fumarate | 0.0-4.0* | 6.9-52.2 (23.67) | 100-1,000 | No | ||||
| Dehydroxy-ascorbate | 2.3-12.6* | Not measured | 10-100 | No | ||||
| Spermine | 0.01-0.13* | 0.04-97.0 (1.6) | 100-1,000 | Yes, ≥500 μM | ||||
| Spermidine | 0.07-10.3* | 0.1-47.5 (2.0) | 100-1000 | Yes, ≥500 μM | ||||
**
All metabolites in this table were tested for PMN priming as a proof of concept. Only succinate, spermine and spermidine primed the PMN oxidase.
Fold Change=Median ARDS/Median No ARDS; p-value=Mann Whitney 2-tailed t-test
*
Obtained through the Human Metabolome Database (http://www.hmdb.ca/metabolites/HMDB00254)