FIGURE 2.

Adoptive transfer of MCMV immune or nonimmune sera into D+/R− μMT transplants.

(A) Sera from uninfected (MCMV− Ig) or MCMV infected (MCMV+ Ig) C57BL/6 mice were pooled and anti-MCMV antibody titers measured by ELISA at 450 nm optical density (O.D.). (B-I). D+/R− transplants were performed using μMT recipients, which were treated post-transplant with no Ig, MCMV− Ig, or MCMV+ Ig (n=3/experimental group). (B, C) Western blots of allograft lysates at post-transplant day 3 were probed using anti-mouse IgG and anti-actin antibodies (B), and the IgG:actin ratio quantitated (C) using Image J. (D-G) Allografts from μMT recipients at post-transplant day 3 were stained for IgG-AF488 (D) or C3-FITC (F). Representative images of cortex (upper panels) and medulla (lower panels) are shown, with brightfield overlay to orient tissue morphology (60x; white bar=50 μm). Immunostaining for IgG (E) or C3 (G) was quantitated using Image J as in Figure 1, and compared between groups receiving no Ig, MCMV− Ig, or MCMV+ Ig. (H-I) Allografts at day 14 post-transplant were stained using hematoxylin and eosin (H) and damage scores quantitated (I).

* p<0.05; ** p<0.01; *** p<0.001.