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. Author manuscript; available in PMC: 2022 Aug 1.
Published in final edited form as: Transplantation. 2021 Aug 1;105(8):1718–1729. doi: 10.1097/TP.0000000000003548

FIGURE 3.

FIGURE 3.

IgG deposition in allografts with immunosuppression.

(A) Allografts of cyclosporine-immunosuppressed MCMV D−/R−, D+/R− and D+/R+ recipients were stained with IgG-AF488 at day 14 post-transplant (n=5–6/group). Kidneys from non-transplant C57BL/6 mice (“No Tx”) served as staining controls (left panels, n=3). Representative images of cortex (upper panel) and medulla (lower panel) are shown (40x; white bar=50 μm). (B) Fluorescence immunostaining was quantitated for 10 HPF per kidney using Image J and compared between groups. (C) MCMV− Ig, MCMV+ Ig, or sera from immunosuppressed D−/R−, D+/R− or D+/R+ transplant recipients were analyzed by ELISA for anti-MCMV antibodies at OD450 (O.D). (D) Serologic anti-MCMV antibody levels (O.D.) were correlated with allograft IgG immunostaining for D+/R− and D+/R+ transplant recipients.

* p<0.05; *** p<0.001.