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. 2021 May 4;8:627022. doi: 10.3389/fmolb.2021.627022

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Copyright © 2021 Islam Khan and Law.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CASC9 expressions in colon cell lines and effective silencing by Dicer-substrate siRNA. (A) The expression of CASC9 in CRC cell lines DLD-1, HT-29, SW480, and HCT-116 was compared with normal colon cell line CCD-112CoN using RT-qPCR. The data are shown as mean ± SEM of eight independent experiments. (B,C) Effective and consistent silencing of CASC9 by Dicer-substrate siRNA techniques was observed. The data are shown as mean ± SEM compared to the negative control Dsi-NC. n = 6, ***P < 0.001.