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Silencing of CASC9 enhanced autophagy in CRC cells. The expressions of autophagy marker proteins LC3B and p62 were measured by Western blotting in HCT-116 (A,B) and SW480 (C,D) cells. After Dsi-CASC9–mediated silencing, the ratio of autophagy marker LC3B-II to LC3B-I significantly increased in HCT-116 and SW480 cells with corresponding decrease in p62 expression. The data are shown as mean ± SEM using β-actin as housekeeping control (*P < 0.05, **P < 0.01, and n = 4).
