FIGURE 5.

Silencing of CASC9 promoted the AMPK signaling pathway but downregulated the AKT and mTOR pathways. The ratio of p-AMPKα/AMPKα, p-AKT/AKT, and p-mTOR/mTOR to GAPDH were evaluated by Western blotting in HCT-116 (A–D) and SW480 cells (E–H). Dsi-CASC9 significantly promotes AMPK signaling in HCT-116 and SW480 cells compared to Dsi-NC. In contrast, CASC9 silencing significantly downregulated AKT and mTOR signaling pathways in HCT-116 and SW480 cells. The data are shown as mean ± SEM using GAPDH as housekeeping gene (*P < 0.05, and n = 3).