FIGURE 6.

CASC9 silencing altered the expressions of EMT marker proteins in CRC cells. The EMT markers E-cadherin, N-cadherin, and vimentin were evaluated by Western blotting in HCT-116 (A–D) and SW480 cells (E–H). In both cell lines, CASC9 significantly upregulated E-cadherin and downregulated vimentin expressions. The N-cadherin expression was also downregulated but did not reach statistical significance in HCT-116 cells. The data are shown as mean ± SEM using GAPDH as housekeeping gene (*P < 0.05, and n = 3).