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. 2021 May 17;12:2869. doi: 10.1038/s41467-021-23161-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a, b MS spectra of lipids from a single MDA-MB-231cell without (a) and with (b) photochemical derivatization (the m/z values in blue: PB products of lipids). c Total ion chromatogram (TIC) of a single MDA-MB-231 cell sampled 9 times by on-demand DAESI-MS after cell fixation and photochemical derivatization. d Average MS spectra of the 1st, 8th, and 9th samplings (the m/z values in red: PB products of lipids). e Pairwise correlation coefficients calculated using the intensities of PB product ions of each spray. Source data are provided in a Source Data file.