Fig. 2. PIP₂ binding sites in the AENTH tetramer.

a PIP₂ binding site shared by the ANTH and ENTH domains. b PIP₂ binding site within the ENTH domain. c PIP₂ binding site in the interface between two ENTH domains. The residues involved in PIP₂ binding are shown in sticks and colored blue for nitrogen and gray and cyan for carbon in ENTH and ANTH, respectively. The polar head of the PIP₂ is shown in stick format and colored red, orange, and light gray for oxygen, phosphate, and carbon, respectively with the corresponding density shown as a transparent surface.