Fig. 5. Protein-lipid interactions of the AENTH 16-mer complex structure and of the ANTH-ENTH assembly on GUVs.

a, left, All the polar heads from the PIP₂ placed in the structure (shown in sticks) are contained in the region near to the core of the structure (shown in surface representation), indicating the presence of a PIP₂ micelle in the center of the map. Right, only the α0 helices from ENTH subunits are shown in cartoon representation (in pink) together with the PIP₂ shown in stick (colored by atom), all of them are pointing towards the interior of the structure. b–e Flexible fitting of the tetrameric model to the previously obtained cryo-EM map of ANTH-ENTH coat on GUV tubules¹⁸. b Overview of the GUVs tubular coat structure with the tetramer fitting inside the lobes of the structure (EM-DB entry: EM-2896). Bottom, close-up view of the tetramer fitted into the EM density of the GUV structure. c AENTH tetramer structure fitted to the GUVs coat structure. The regions involved in PIP₂ contacts are colored in gold in the cartoon representation. The bilayer plane is indicated as a gold dashed line. d AENTH tetramer in the 16-mer ANTH-ENTH complex, with the lipid-binding regions highlighted in gold in the cartoon representation. e Close-up for the ANTH lysine patch residues involved in the coordination of the polar head of PIP₂ lipid.