Fig. 6. Effect of AENTH tetramer and mutants on GUVs by fluorescence microscopy.

a, b ANTH-GFP and ENTH-GFP do not bind GUVs without PIP₂. c, d ANTH-GFP or ENTH-GFP mixed with ENTH or ANTH, respectively, caused membrane deformation as seen by the numerous membrane protrusions around GUVs. e, f ANTH-GFP or ENTH-GFP did not cause a remodeling effect on the GUV membrane. g–n ANTH and ENTH mutants mixed with ENTH-GFP and ANTH-GFP, respectively, showed different membrane deformation capabilities. Each experiment was repeated independently 3 times with similar results. All scale bars are 5 µm.