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. 2021 May 17;12:2889. doi: 10.1038/s41467-021-23151-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a LUVs observed by negative staining. b ANTH does not deform LUVs notably. c. ENTH causes LUVs to become aggregated and to adopt irregular shapes. d ANTH together with ENTH reshapes LUVs into tubular structures as previously reported¹⁸. e–l ANTH and ENTH mutants show different effects on LUVs. ANTH R25A (e), ENTH E54A/D57A/D60A (k) and ANTH R3A/I4A/D37A/H38A (l) mutants (mixed with its wild type partner domain) display an AENTH wt phenotype reshaping LUVs into tubular structures ANTH R29A (f) and ENTH Y100R (g) ANTH K10D/K13D/K14D (i) and ANTH K10D/K13D (j) mutants (mixed with its wild type partner domain) display an ENTH wt phenotype being unable to tubulate LUVs. Each experiment was repeated independently 3 times with similar results. All scale bars are 200 nm.