Table 1.
Experimental and theoretical masses for ANTH, ENTH, PIP2, and adducts/complexes observed by native MS.
| Species | Ligand or adduct | Theoretical mass (Da) | Experimental mass (Da) | Main charge state | FWHMa |
|---|---|---|---|---|---|
| – | diC8-PI(4,5)P2 | 747.5 | – | – | – |
| ENTH | – | 18847.9 | 18847.8 | 8+b | 1.2 |
| +Na | 18870.9 | 18871.4 | 8+b | 1.1 | |
| +PIP2 | 19594.4 | 19593.2 | 8+b | 1.1 | |
| ANTH | – | 33210.0 | 33209.6 | 11+ | 1.2 |
| +Na | 33233.0 | 33230.3 | 11+ | 1.3 | |
| ANTH + PIP2 | 33956.5 | 33959.4 | 11+ | 1.3 | |
| ANTH8:ENTH8 (wildtype) | +22 PIP2 | 433181 | 432887 | 41+ | 9.7 |
| +23 PIP2 | 433892 | 433633 | 41+ | 10.5 | |
| +24 PIP2 | 434627 | 434380 | 41+ | 9.2 | |
| ANTH6:ENTH6 (ANTH R3A-I4-D37R-H38A) | +17 PIP2 | 324125 | 324382 | 36+ | 8.5 |
| +18 PIP2 | 324872 | 325141 | 36+ | 10.7 | |
| +19 PIP2 | 325618 | 325902 | 36+ | 12.2 |
aFWHM (full width at half maximum) is given for the predominant charge state peak to indicate the experimental error.
bSensitivity for the Orbitrap UHMR instrument used here is reduced at low m/z (< 2500), so higher charge states of free ENTH may be attenuated.