Table 2.
Cryo-EM data collection, refinement, and validation statistics of the AENTH 16-mer assembly.
| Data collection and processing | |
|---|---|
| Magnification | ×75,000 |
| Voltage (kV) | 300 |
| Electron exposure (e–/Å2) | 75.2 |
| Defocus range (μm) | −1 to −3 |
| Pixel size (Å) | 1.065 |
| Symmetry imposed | D2 |
| Initial particle images (no.) | 195,536 |
| Final particle images (no.) | 79,414 |
| Map resolution (Å) | 3.9 |
| FSC threshold | 0.143 |
| Map resolution range (Å) | 3.7 to 5.7 |
| Refinement | |
| Initial model used | 5onf, 5oo7 |
| Model resolution (Å) | 3.9 |
| FSC threshold | 0.5 |
| Model resolution range (Å) | – |
| Map sharpening B factor (Å2) | −200 |
| Model composition | |
| Non-hydrogen atoms | 26,192 |
| Protein residues | 3152 |
| Ligands | 20 |
| B factors (Å2) | |
| Protein | 70 |
| Ligand | – |
| r.m.s. deviations | |
| Bond lengths (Å) | 0.012 |
| Bond angles (°) | 2.037 |
| Validation | |
| MolProbity score | 1.91 |
| Clashscore | 2.60 |
| Poor rotamers (%) | 3.78 |
| Ramachandran plot | |
| Favored (%) | 93.56 |
| Allowed (%) | 5.80 |
| Disallowed (%) | 0.64 |