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. 2021 May 17;11:10418. doi: 10.1038/s41598-021-89853-6

Figure 2.

Figure 2

Effect of temperature on diffusion of nuclear dye Hoechst 33342 into pancreatic islets. (a) Staining profiles of pancreatic islets incubated with Hoechst 33342 for 15 min, 6 h or 24 h at room temperature (n = 6–7). (b) Staining profiles of pancreatic islets incubated with Hoechst 33342 for 15 min, 6 h or 24 h at 37 °C (n = 6–7). (c) Pancreatic islet stained with FDA (green), PI (magenta) and Hoechst 33342 (white) for 6 h at 37 °C imaged a confocal microscope. (d) Pancreatic islet stained with FDA (green) and Hoechst 33342 (white) for 6 h at 37 °C imaged on a two-photon microscope. (e) Pancreatic islet stained with FDA (green), PI (magenta) and Hoechst 33342 (white) for 6 h at 37 °C imaged on a light sheet microscope. (f) 3D-reconstruction from multi-angle imaging (4 angles, 90° apart) of pancreatic islet stained with FDA (green), PI (magenta) and Hoechst 33342 (white) using light sheet microscopy. Representative images shown. Figures (a) and (b) were produced in GraphPad Prism (version 8.4.1, https://www.graphpad.com). In (c–a), each slice is labelled with distance in μm from the top of the islet. FDA fluorescein diacetate, PI propidium iodide.