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. 2021 May 4;12:586886. doi: 10.3389/fmicb.2021.586886

FIGURE 8.

FIGURE 8

RNA1 is essential for RNase J-deficient plasmid-loss. (A) Both pVG1 and pVG1[PRNA1*] are able to establish themselves in RN4220 (an S. aureus strain which does not harbor any plamids), although the colonies are visibly smaller for pVG1[PRNA1*]. pVG1[PRNA1*] is able to establish itself in the ΔrnjA strain to form colonies in 24 h. The RN4220 and the ΔrnjA colonies are from two different MHC-plates. (B) The colony counts from MHC-plates (pVG1 and pVG1[PRNA1*] transformants) at ∼24 h were normalized with counts from MHT-plates (pCN36 transformants, counted at ∼42 h). RN4220 exhibits similar colony, colony ratios for pVG1 and pVG1[PRNA1*] (blue and green bars, respectively), whereas only pVG1[PRNA1*] replicates rapidly in ΔrnjA to form colonies within 24 h. Note that ΔrnjA transformed with pVG1 will eventually form colonies when the plates are left long enough in the incubator.