Figure 5.

KLF5 and HIF1α restored the attenuated proliferation of PASMCs affected by miR-320-3p. PASMCs were transfected with control mimics or miR-320-3p mimics for 12 hr., and then transfected with pcDNA3.1, KLF5 plasmid or HIF1α plasmid. A. Proteins related to proliferation (PCNA and Survivin) were determined by western blotting. B. The PASMC viability was detected by CCK-8 tests. C, D. The PASMC proliferation ability was measured by EdU proliferation assay. *P < 0.05, **P < 0.01, ***P < 0.005.