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. 2021 Apr 15;13(4):2021–2040.

Figure 5.

Figure 5

LincRNA-p21 promoted autophagy by suppressing STAT3 signalling. A. Immunostaining images showed LC3 intensity and DAPI signal in cells transfected with the lincRNA-p21 or control vector following LPS treatment. The bar graph quantified the number of LC-3 positive autophagosomes. LPS alone decreased the number of LC3 puncta while lincRNA-p21 overexpression recovered the number. B. The protein levels of LC3-I, LC3-II, beclin-1, and p62 in cells transfected with the lincRNA-p21 or control vector following LPS treatment were measured by western blotting. Bar graphs quantified the protein levels. LPS alone diminished the LC3II/LC3I ratio and Becline-1 level but increased p62 level while lincRNA-p21 overexpression reversed those changes. C. The protein levels of p-STAT3 in cells transfected with the lincRNA-p21 or control vector following LPS treatment were examined by western blotting. The bar graph quantified the protein levels. LPS treatment alone increased pSTAT3 level while lincRNA-p21 overexpression suppressed the increase. n = 3; *P < 0.05; **P < 0.01; ***P < 0.001.