Figure 8.

Bilobalide inhibited neuroinflammation and promoted autophagy in vivo. A. The mRNA levels of TNF-α, IL-6 and IL-1β in different groups of mice were measured by qRT-PCR. APP/PS1 mice had higher levels compared to WT while bilobalide treatment downregulated those levels. B. The protein levels of LC3-I, LC3-II, beclin-1, and p62 in different groups of mice were measured by western blotting. Bar graphs quantified the protein levels. Compared with WT mice, APP/PS1 mice had lower levels of LC3II/LC3I ratio and Becline-1 but higher level of p62 while bilobalide treatment reversed those changes. C. The protein levels of p-STAT3 in different groups of mice were analysed by western blotting. Bar graph quantified the protein level. Compared with WT mice, APP/PS1 mice had higher level while bilobalide treatment reduced p-STAT3 level. D. LincRNA-p21 levels in different groups of mice were analysed by qRT-PCR. Compared with WT mice, APP/PS1 mice had lower lincRNA-p21 level while bilobalide treatment recovered the level. n = 6 for each group; *P < 0.05; **P < 0.01; ***P < 0.001.