Table 1.

Primer sequences for the RT-PCR assay

Genes		Sequences	Length (bp)
ALP	Forward	5’-CCTTGAAAAATGCCCTGAAA-3’	191
	Reverse	5’-CTTGGAGAGAGCCACAAAGG-3’
RUNX-2	Forward	5’-GAGCTACGAAATGCCTCTGC-3’	173
	Reverse	5’-GGACCGTCCACTGTCACTTT-3’
COL-1	Forward	5’-TGGTCCTCAAGGTTTCCAAG-3’	123
	Reverse	5’-TTACCAGCTTCCCCATCATG-3’
OCN	Forward	5’-CATGAGGACCCTCTCTCTGC-3’	153
	Reverse	5’-AGGTAGCGCCGGAGTCTATT-3’
actin	Forward	5’-GCGACCTCACCGACTACCT-3’	136
	Reverse	5’-GCCATCTCGTTCTCGAAGTC-3’