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. 2021 May 17;12:2887. doi: 10.1038/s41467-021-23240-7

Fig. 4. Idh2 upregulation prevents impaired fatty acid oxidation associated with Yap knockdown.

Fig. 4

a Graph showing the changes in chromatin accessibility and transcript abundance at enhancers or promoters (TSS). Numbers within bars indicate the number of genes detected in each comparison (FDR < 0.05, rank-MANOVA), (b) sequencing tracks of Idh2 transcript in AAV6:lacZ-shRNA (blue) and AAV:Yap shRNA (red). Each track represent a biological replicate from an individual animal (n = 3), (c) incomplete fatty acid oxidation in EDL muscles analysed 2 weeks after injection of control, AAV6:Yap-shRNA, AAV6:Idh2 vectors or both (n = 10 biologically independent animals, mean ± SEM, * shows significant difference, one-way ANOVA with Tukey’s multiple comparison test with exact p values provided in source data), (d) representative western blots of Yap, Idh2, and total protein levels in TA muscles from mice treated as in (c) (n = 5 biologically independent animals), (e) Representative H and E images of TA muscle from mice treated as in (c) (n = 5 biologically independent animals, scale bars indicate 50 µm).