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. 2021 May 17;12:2887. doi: 10.1038/s41467-021-23240-7

Fig. 5. Increasing Yap in the musculature of obesogenic db/db mice enhances energy expenditure to reduce adiposity and hepatic steatosis.

Fig. 5

a Representative image of db/db mice 10 weeks after administration of AAV6:empty vector or AAV6:Yap vectors (n = 7 db/db empty vector, 9 db/db Yap, 8 db/+) (b) body mass and (c) fat mass (in grams) of db/db mice over 14 weeks following administration of AAV6:empty vector (black) or AAV6:Yap (green) vectors. db/+ mice treated with AAV6:empty vector included as a control (red) (mean ± SEM, *indicates p < 0.05 with exact p values provided in source data, n = 7 db/db empty vector, 9 db/db Yap, 8 db/+, two-way ANOVA with Tukey’s multiple comparisons test)), (d) average daily food intake (g = grams) of mice treated as in (b) after 12 weeks (n = 6 db/db empty vector, 8 db/db Yap, 8 db/+, mean ± SEM, * indicates p < 0.05 with exact p values provided in Source Data, one-way ANOVA with Tukey’s multiple comparisons test), (e) fasting plasma insulin concentrations (ng/ml) of mice treated as in b) after 14 weeks of treatment (n = 6 db/db empty vector, 7 db/db Yap, 8 db/+, mean ± SEM, *indicates p < 0.05 with exact p values provided in source data, one-way ANOVA with Tukey’s multiple comparisons test), (f) plasma insulin concentrations in mice treated as in (b) after 12 weeks following 6 h fast (0 min) or oral gavage of glucose (15 min) (n = 6 db/db empty vector, 7 db/db Yap, mean ± SEM, * indicates p < 0.05 with exact p values provided in source data, one-way ANOVA with Sidak’s multiple comparisons test), (g–h) Energy expenditure (kcal/hr/kg lean body mass) of mice treated as in (b) after 10 weeks of treatment. 12 h inactive and 12 h active cycles are indicated in (g) with quantification shown in (h) (n = 6 db/db empty vector, 4 db/db Yap, mean ± SEM, t test comparing empty vector and Yap treatments in inactive (p = 0.03) or active phases (p = 0.009), (i) representative H and E image of adipose tissue, (j) Ucp-1 labelling of inguinal adipose tissue. Insert shows higher magnification, (k) livers of mice stained with H and E following treatment as in (b) (i–k n = 6 db/db empty vector, 8 db/db Yap, scale bars indicate 50 µm). All n values biologically independent animals.