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. Author manuscript; available in PMC: 2021 Dec 17.
Published in final edited form as: Biochem Biophys Res Commun. 2020 Sep 29;533(4):879–885. doi: 10.1016/j.bbrc.2020.09.058

Fig. 1. Some progestogens display different efficacies and potencies via PR-B.

Fig. 1.

COS-1 cells transiently transfected with (A) 900 ng pSG5-empty vector or (B-D) pSG5-hPR-B expression vector and 9000 ng pTAT-2xPRE-E1b luciferase reporter (transfection condition #1), were treated with 0.1% EtOH (vehicle) or increasing concentrations of each ligand for 24 hours. Luciferase activity was measured and normalized to protein concentration. (A-B) Relative luciferase activity is shown with PR-B R5020 (maximal response) set as 100% and all other response relative to this. The shaded bars in B indicate the reported serum concentrations of progestogens in women. (C) Efficacy and (D) −logEC50 values ± SEM of the ligands via PR-B were plotted and analysed using one-way ANOVA with a Bonferroni post-test. Different letters denote statistically significant differences while the same letters do not.