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. 2021 May 4;9:660424. doi: 10.3389/fchem.2021.660424

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Copyright © 2021 Ibba, Piras, Corona, Riu, Loddo, Delogu, Collu, Sanna, Caria, Dettori and Carta.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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N¹,N⁵-Bis(4-(5,6-dichloro-1H-benzo [d][1,2,3]triazol-1-yl)phenyl)glutaramide (3b) induced apoptosis in human lung carcinoma SK-MES 1 cells. The percentage of live, apoptotic, and necrotic cells were measured by flow cytometry using the PI-annexin V assay. Dot plots show cell death in SK-MES1 cells: control (A), treated cells with 20 µM (B), treated cells with 7 µM (C). Percentage of live, apoptotic, and necrotic cells (D,E). Each value represents the mean ± SD of independent experiments (n = 3).