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. 2021 May 4;12:640672. doi: 10.3389/fimmu.2021.640672

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Copyright © 2021 Euchner, Sprissler, Cathomen, Fürst, Schrezenmeier, Debatin, Schwarz and Felgentreff

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of generated NK cells for expression of maturity markers and NK-cell receptors. Representative flow cytometry analyses for the expression of (A) maturity markers DNAM-1, CD69, CD62L, CXCR1, and CD57; (B) natural cytotoxicity receptors (NCR), and (C) killer cell immunoglobulin-like receptors (KIR) are shown on CD45⁺CD3^-CD56⁺ NK cells obtained at w3. On the right-hand side, expression levels of investigated surface markers gated on CD45⁺CD3^-CD56⁺ NK cells are summarized in whisker plots (A–C) (n=3), and compared to NK cells obtained from culture on OP9-DL1 feeder cells treated with MMC.