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. 2021 May 4;12:669889. doi: 10.3389/fimmu.2021.669889

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Copyright © 2021 West, Mizoro, Wood, Ince, Iversen, Jørgensen, Nome, Sandve, Martin, Loudon and Hazlerigg

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparative abundance of cell clusters at different sampling points. (A) Experimental design. Fish were kept in constant light (LL) from hatching then transferred to short photoperiod (SP; 8L:16D) for 8 weeks before being returned to constant light (LL) for 8 weeks. Finally the fish were transferred to sea water for 24h. Sample points are indicated T1-T4. (B) Subset of cell clusters from T2, T3 or T4 (orange and blue dots) overlaid on T1 cells (grey dots). (C) Increasing abundance of sea-water mitochondrion-rich cells (MRCs SW) and vascular cells (VC 3) during smoltification (D) Decreasing abundance of leukocytes and immune-associated cells during smoltification.