Figure 2.

SNHG9 inhibits Nthy-ori-3 cell autophagy through PTC exosomes. (A, B) SNHG9 was significantly upregulated in K-1 and TPC-1 cell exosomes after overexpressing SNHG9 in PTC cells. (C, D) SNHG9 was significantly downregulated in K-1 and TPC-1 cell exosomes after knocking down SNHG9 in PTC cells. (E, F) Overexpressing SNHG9 in HBSS treated Nthy-ori-3 cells could inhibit cell autophagy detected by mRFP/mCherry-GFP-LC3B autophagy flow. Yellow spots indicate autophagosomes and red spots indicate autophagic lysosomes. (G) Autophagy-related protein P62 was significantly increased after overexpressing SNHG9 in HBSS treated Nthy-ori-3 cells by Western blot. (H and I) K-1^SNHG9-O/K-1^SNHG9-KD exosomes could inhibit/promote Nthy-ori-3 cells autophagy detected by mRFP/mCherry-GFP-LC3B autophagy flow. Yellow spots indicate autophagosomes and red spots indicate autophagic lysosomes. (J) P62 was significantly increased in K-1^SNHG9-O exosome treated Nthy-ori-3 and was downregulated in K-1^SNHG9-KD exosome treated Nthy-ori-3 cells by Western blotting. (K, L) The impact of K-1^SNHG9-O/K-1^SNHG9-KD exosomes on Nthy-ori-3 cell autophagy was decreased after K-1 cell exosomes secretion was inhibited by GW4869. (M) P62 protein change after K-1 cell exosomes secretion was inhibited by GW4869. ***P < 0.001, **P < 0.01, data were pooled from three independent experiments. PTC, papillary thyroid cancer; KD, knock down; O, overexpression. HBSS, Hank’s balanced salt solution.