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. 2021 May 4;11:647034. doi: 10.3389/fonc.2021.647034

Figure 5.

Figure 5

SNHG9 interferes with YBOX3 and inhibits P21 expression through PTC exosomes and regulates Nthy-ori-3 cell autophagy and apoptosis. (A) SNHG9 overexpression could inhibit YBOX3 and P21 protein expression level in both the cytoplasm and nucleus tested by Western blotting. The intracellular reference was GAPDH. The internal nuclear reference was H3. (B) P21 mRNA expression was decreased by SNHG9 overexpression in Nthy-ori-3 cell tested by qPCR. (C, D) Both P21 and YBOX3 protein expression were downregulated in cytoplasm and nucleus of Nthy-ori-3 cell after transfection of small interfering RNA for YBOX3. (E) P21 mRNA expression was decreased in Nthy-ori-3 cell after transfection of small interfering RNA for YBOX3. (F) Both P21 and YBOX3 protein expression were downregulated in cytoplasm and nucleus of Nthy-ori-3 treated with exosomes of K-1_SNHG9-O. (G) P21 mRNA expression was decreased in Nthy-ori-3 cell treated with exosomes of K-1_SNHG9-O. (H) Apoptosis pathway related protein expression in Nthy-ori-3 cells overexpressing SNHG9 or treated with K-1_SNHG9-O. (I) Autophagy pathway related protein expression in Nthy-ori-3 cells overexpressing SNHG9 or treated with K-1_SNHG9-O. ***P < 0.001, data were pooled from three independent experiments. PTC, papillary thyroid cancer; O, overexpression; qPCR, quantitative polymerase chain reaction.