Figure S3.

yki is dispensable in outer BCs for migration. (A) Graph showing mean migration ± SEM derived from means of each replicate superimposed on violin plots of migration measurements for each of the indicated genotypes. Replicate trials consisted of n ≥ 20 egg chambers for each genotype. (B) Confocal micrograph of an egg chamber with outer BCs coexpressing GFP (green) under the control of slbo-GAL4, together with dsRNA for yki. F-actin labeled by phalloidin in red and DNA with TO-PRO-3 in blue. (C) Dot plots showing quantification of area under curve for F-actin and Crb staining at the back, middle, and front (BMF) of the BC cluster derived from line scans taken through clusters. Error bars indicate SEM of replicates (n = 20 clusters). (D) Confocal image (as in B) but stained with Crb instead of F-actin. Significance determined by unpaired two-tailed Student’s t test.