Video 4.
Time-lapse movie of abnormal BC migration (10 frames/s) showing early tumbling of the cluster and multidirectional actin protrusions in not1 cells labeled with nuclear GFP using MARCM. In this example of a cluster that fails to delaminate, the percent of protrusions at front, side, and back are 13.1, 79.5, 7.4, respectively. The tumbling index is 100%. F-actin is labeled with LifeAct-GFP. Egg chamber genotype: hsFLP, tub-Gal4, UAS-GFP/+; slbo-LifeAct-GFP/+; not1, FRT80B/tub-Gal80, FRT80B.