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. 2021 May 14;220(6):e202007172. doi: 10.1083/jcb.202007172

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© 2021 Yang et al.

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Figure S3. — I154 and L158 are required for endophilin A1 binding to calmodulin. (A) Binding of EEN1 WT and mutants to GST-calmodulin in pull-down assay. Bound proteins were analyzed by SDS-PAGE and immunoblotting with anti-His antibodies. (B) Quantification of mutants binding to GST-calmodulin in A. Data are represented as mean ± SEM, n = 5 independent experiments. P values were calculated using one-way ANOVA by Dunnett post hoc test.