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Spatiotemporal relationship between spine membrane retraction and increase in F-actin in EEN1fl/fl spines before and during glycine-induced sLTP. Decrease in fluorescent signals of spine membrane (labeled by mGFP) and increase in F-actin (labeled by LifeAct-mCherry) in sequential differential images are color coded green and red, respectively. Video plays at 7 frames/s. Scale bar, 2 µm.
