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Spatiotemporal relationship between spine membrane expansion and increase in F-actin in EEN1 KO spines before and during glycine-induced sLTP. Increases in fluorescent signals of spine membrane (labeled by mGFP) and F-actin (labeled by LifeAct-mCherry) in sequential differential images of EEN1fl/fl mouse hippocampal neuron coexpressing mGFP-2A-Cre and LifeAct-mCherry are color coded green and red, respectively. Video plays at 7 frames/s. Scale bar, 2 µm.
