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W-7 inhibits ionomycin-induced accumulation of endophilin A1 at the cell periphery in Neuro-2a cells. Neuro-2a cells transiently transfected with construct expressing EGFP-EEN1 were treated with W-7 (20 µM) for 20 min and imaged by confocal microscopy every minute for 2 frames, followed by ionomycin (1 µM) addition and continued imaging every 10 s to 1 min for 25 frames. Video plays at 7 frames/s. Scale bar, 10 µm.
