Table 4.

Primer and probe sequences for SARS-CoV-2 (N1 and N2 targets) and OC43

Target	Primer/probe sequences	Ref.
SARS-CoV-2 N1	F: 5’-GAC CCC AAA ATC AGC GAA AT-3’ R: 5’-TCT GGT TAC TGC CAG TTG AAT CTG-3’ P: 5’-FAM-ACC CCG CAT TAC GTT TGG TGG ACC-BHQ1-3’ Pdouble: FAM-ACC CCG CAT/ZEN/TAC GTT TGG TGG ACC-3IABkFQ	2019-nCoV CDC EUA kit, IDT Catalog No. 10006606
SARS-CoV-2 N2	F: 5’-TTA CAA ACA TTG GCC GCA AA-3’ R: 5’-GCG CGA CAT TCC GAA GAA-3’ P: 5’-FAM-ACA ATT TGC CCC CAG CGC TTC AG-BHQ1-3’ Pdouble: FAM-ACA ATT TGC/ZEN/CCC CAG CGC TTC AG-3IABkF	2019-nCoV CDC EUA kit, IDT Catalog No. 10006606
OC43	F: 5’-CGATGAGGCTATTCCGACTAGGT-3’ R: 5 ‘-CCTTCCTGAGCCTTCAATATAGTAACC-3’ P: 5’−6FAM-TCCGCCTGGCACGGTACTCCCT-BHQ-3’	Dare, R.K. et al. J Infect. Diseases, 2007, 196: 1321–8