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. Author manuscript; available in PMC: 2022 Jan 15.
Published in final edited form as: ACS Synth Biol. 2020 Dec 30;10(1):132–144. doi: 10.1021/acssynbio.0c00500

Fig. 7.

Fig. 7

The performance of hybrid promoters with yveF and yveG. (a) The design of hybrid promoters. From P1-FG to P8-FG, PadR-1 and PadR-2 were placed before −35 region or −10 region simultaneously or respectively. The P9-FG and P10-FG contained only PadR-1 and PadR-2, respectively. Because the PadR-2 overlapped with the −10 region, and this may affect the promoter performance, we designed another hybrid promoter P11-FG by keeping the −10 sequence from PL. For hybrid promoters P12-FG and P13-FG, they contained both PadR binding boxes, but in P12-FG the −10 sequence from PpadC was used and in the P13-FG the −10 sequence from PL was used (b) The hybrid promoters designed according to PLlacO1 were rested by 0 and 600 mg/L p-coumaric acid. (c) The hybrid promoters, which were designed based on the relative position in PpadC, were tested by 0 and 600 mg/L p-coumaric acid. All data are reported as mean±s.d. from three independent experiments (n=3). Error bars are defined as s.d.