Table 1.
List of strains and plasmids used in this study
| Strains | Genotype | Reference |
|---|---|---|
| XL 1-Blue | recA1 endA1gyrA96thi-1hsdR17supE44relA1lac [F’ proAB lacIqZDM15n1O (TetR)] | Stratagene |
| BW25113(F’) | rrnBT14 ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD78 F’ [traD36 proAB lacIqZΔM15 Tn10(Tetr)] | Yale CGSC |
| Plasmids | Description | Reference |
| pZE12-luc | pLlacO-1; luc; ColE1 ori; AmpR | 20 |
| pCS27 | pLlacO-1; p15A ori; KanR | 21 |
| pZE-PpadC-egfp | pZE12-luc carrying PpadC from Bacillus subtilis and egfp | In this study |
| pZE-PpadC-yveF-egfp | pZE12-luc carrying PpadC and yveF from Bacillus subtilis and egfp | In this study |
| pZE-Ppadc-yveG-egfp | pZE12-luc carrying PpadC and yveG from Bacillus subtilis and egfp | In this study |
| pZE-PpadC-yveF-yveG-egfp | pZE12-luc carrying PpadC and yveF and yveG from Bacillus subtilis and egfp | In this study |
| pCS-lpp1.0-PadR | pCS27 carrying promoter lpp1.O and PadR from Bacillus subtilis | In this study |
| pCS-lpp0.8-PadR | pCS27 carrying promoter lpp0.8 and PadR from Bacillus subtilis | In this study |
| pCS-lpp0.5-PadR | pCS27 carrying promoter lpp0.5 and PadR from Bacillus subtilis | In this study |
| pCS-lpp0.2-PadR | pCS27 carrying promoter lpp0.2 and PadR from Bacillus subtilis | In this study |
| pCS-lpp0.2-K37A | pCS27 carrying promoter lpp0.2 and PadR mutant K37A from Bacillus subtilis | In this study |
| pCS-lpp0.2-H38A | pCS27 carrying promoter lpp0.2 and PadR mutant H38A from Bacillus subtilis | In this study |
| pCS-lpp0.2-S39A | pCS27 carrying promoter lpp0.2 and PadR mutant S39A from Bacillus subtilis | In this study |
| pCS-lpp0.2-Q40A | pCS27 carrying promoter lpp0.2 and PadR mutant Q40A from Bacillus subtilis | In this study |
| pCS-lpp0.2-Q61A | pCS27 carrying promoter lpp0.2 and PadR mutant Q61A from Bacillus subtilis | In this study |
| pCS-lpp0.2-K64A | pCS27 carrying promoter lpp0.2 and PadR mutant K64A from Bacillus subtilis | In this study |
| pZE-P1-yveF-yveG-egfp | pZE12-luc carrying PpadC mutant P1, and yveF and yveG from Bacillus subtilis and egfp | In this study |
| pZE-P2-yveF-yveG-egfp | pZE12-luc carrying PpadC mutant P2, and yveF and yveG from Bacillus subtilis and egfp | In this study |
| pZE-P3-yveF-yveG-egfp | pZE12-luc carrying PpadC mutant P3, and yveF and yveG from Bacillus subtilis and egfp | In this study |
| pZE-P4-yveF-yveG-egfp | pZE12-luc carrying PpadC mutant P4, and yveF and yveG from Bacillus subtilis and egfp | In this study |
| pZE-P5-yveF-yveG-egfp | pZE12-luc carrying PpadC mutant P5, and yveF and yveG from Bacillus subtilis and egfp | In this study |
| pZE-P6-yveF-yveG-egfp | pZE12-luc carrying PpadC mutant P6, and yveF and yveG from Bacillus subtilis and egfp | In this study |
| pZE-P7-yveF-yveG-egfp | pZE12-luc carrying PpadC mutant P7, and yveF and yveG from Bacillus subtilis and egfp | In this study |
| pZE-P8-yveF-yveG-egfp | pZE12-/wc carrying PpadC mutant P8, and yveF andyveG from Bacillus subtilis and egfp | In this study |
| pZE-P9-yveF-yveG-egfp | pZE12-luc carrying PpadC mutant P9, and yveF andyveG from Bacillus subtilis and egfp | In this study |
| pZE-P10-yveF-yveG-egfp | pZE12-luc carrying PpadC mutant P10, and yveF and yveG from Bacillus subtilis and egfp | In this study |
| pZE-P11-yveF-yveG-egfp | pZE12-luc carrying PpadC mutant P11, and yveF and yveG from Bacillus subtilis and egfp | In this study |
| pZE-P12-yveF-yveG-egfp | pZE12-luc carrying PpadC mutant P12, andyveF and yveG from Bacillus subtilis and egfp | In this study |
| pZE-P13-yveF-yveG-egfp | pZE12-luc carrying PpadC mutant P13, and yveF and yveG from Bacillus subtilis and egfp | In this study |
| pZE-P1-egfp | pZE12-luc carrying PpadC mutant P1 and egfp | In this study |
| pZE-P2-egfp | pZE12-luc carrying PpadC mutant P2 and egfp | In this study |
| pZE-P3-egrp | pZE12-luc carrying PpadC mutant P3 and egfp | In this study |
| pZE-P4-egfp | pZE12-luc carrying PpadC mutant P4 and egfp | In this study |
| pZE-P5-egfp | pZE12-luc carrying PpadC mutant P5 and egfp | In this study |
| pZE-P6-egfp | pZE12-luc carrying PpadC mutant P6 and egfp | In this study |
| pZE-P7-egfp | pZE12-luc carrying PpadC mutant P7 and egfp | In this study |
| pZE-P8-egfp | pZE12-luc carrying PpadC mutant P8 and egfp | In this study |
| pZE-P9-egfp | pZE12-luc carrying PpadC mutant P9 and egfp | In this study |
| pZE-P10-egfp | pZE12-luc carrying PpadC mutant P10 and egfp | In this study |
| pZE-P11-egfp | pZE12-luc carrying PpadC mutant P11 and egfp | In this study |
| pZE-P12-egfp | pZE12-luc carrying PpadC mutant P12 and egfp | In this study |
| pZE-P13-egfp | pZE12-luc carrying PpadC mutant P13 and egfp | In this study |
| pSC-lpp1.0-yveF-yveG | pCS27 carrying lpp1.0, yveF, and yveG | In this study |