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. Author manuscript; available in PMC: 2021 May 18.
Published in final edited form as: Exp Cell Res. 2018 Dec 21;375(2):106–112. doi: 10.1016/j.yexcr.2018.12.012

Fig. 1. Combining clotrimazole or bifonazole with romidepsin leads to increased apoptosis.

Fig. 1.

A) HCT-116 cells were left untreated (Control) or treated for 6 h with 25 ng/ml romidepsin alone (RD) or in combination with 25 μM BIF or CTZ (RD+BIF; RD+CTZ). The medium was subsequently removed and cells were incubated in romidepsin-free medium in the absence or presence of CTZ for an additional 42 h, after which cells were stained with APC-labeled recombinant annexin and the DNA dye SYTOX green and assayed by flow cytometry. Cells were also incubated with 25 μM CTZ alone for 48 h (CTZ). The red box denotes annexin-positive cells that were quantitated in subsequent experiments. B) HCT-116, A549, MDA-MB-231, IGROV1 and 786–0 cells were treated as outlined in (A), using 1, 5, or 25 μM CTZ or BIF. The percentages of annexin positive cells were determined and summarized in the graph. At least 3 separate experiments were performed for each condition. C) A549 and HCT116 cells were treated for 48 h with 500 nM or 250 nM belinostat, respectively, alone or in combination with 25 μM CTZ or BIF continuously for 48 h. Cells were then stained with annexin V/SYTOX green and assayed by flow cytometry. Significance (p values) is shown for the bracketed conditions.