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. Author manuscript; available in PMC: 2021 May 18.
Published in final edited form as: Exp Cell Res. 2018 Dec 21;375(2):106–112. doi: 10.1016/j.yexcr.2018.12.012

Fig. 2. Romidepsin in combination with CTZ or BIF induces PARP and caspase cleavage.

Fig. 2.

A549 or HCT-116 cells were treated for 6 h with 25 ng/ml romidepsin (RD) alone or in combination with 25 μM clotrimazole (CTZ) or bifonazole (BIF). Subsequently the medium was removed and cells were incubated in romidepsin-free medium in the absence or presence of CTZ or BIF for an additional 42 h. Cells were also treated with 25 μM CTZ or BIF continuously for 48 h. Cleared lysates were then subjected to PAGE and transferred to nitrocellulose. The blot was probed with antibodies to cleaved caspase 3 and cleaved PARP. GAPDH served as a loading control. Results are from one of 3 independent experiments.