Figure 4. miR-29a-3p depletion blocked the effects of sevoflurane (SEVO) on hepatocellular carcinoma (HCC) progression. The Huh7 and Hep3B cells were transfected with anti-miR-29a-3p or anti-miR-NC (negative control) and then stimulated with 4% SEVO for 48 h. A, miR-29a-3p expression was examined by qRT-PCR (n=3). B and C, MTT assay was employed to assess cell proliferation ability (n=3). D, Flow cytometry was used to determine cell apoptosis rate (n=3). E and F, Transwell assay was employed to assess cell migration and invasion (n=3). G and H, The levels of cell apoptosis-related proteins were detected by western blot assay (n=3). Data are reported as means±SD. *P<0.05 (Student's t-test or ANOVA).
