Figure 5. miR-29a-3p targeted CBX3. A, The interaction between miR-29a-3p and CBX 3'UTR was predicted by online tool Targetscan. B and C, Huh7 and Hep3B cells were treated with sevoflurane (SEVO) for 48 h. The luciferase activity of Huh7-SEVO and Hep3B-SEVO cells transfected with CBX3-WT (wild type) or CBX3-MUT (mutant) and miR-29a-3p or miR-NC (negative control) was examined (n=3). D and E, The mRNA and protein levels of CBX3 were measured in Huh7-SEVO and Hep3B-SEVO cells transfected with miR-29a-3p or miR-NC (n=3). F and G, The mRNA and protein levels of CBX3 were analyzed in hepatocellular carcinoma (HCC) tumor tissues (n=30) and SEVO-treated HCC tumor tissues (n=30). In F, data are reported as median and interquartile range. *P<0.05 (Kruskal-Wallis). H and I, The mRNA and protein levels of CBX3 were detected in Huh7 and Hep3B cells, as well as in Huh7-SEVO and Hep3B-SEVO cells (n=3). J, The association between miR-29a-3p and CBX3 levels was investigated. Data are reported as means±SD. *P<0.05 (Student's t-test or ANOVA).