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. 2021 May 17;13:114. doi: 10.1186/s13148-021-01099-1

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — DNA methylation and gene expression profiles of SOCS3 in control and T2DM individuals. (a) Schematic representation of the CpG sites distribution within the DNA region under investigation in the SOCS3 locus. The image indicates the location and extent of the region analysed in the EpiTYPER assay (filled and empty circles represent the analysed and unanalysed CpG sites, respectively) and the PCR amplicon used in the modified DNA immunoprecipitation assay (mDIP). The arrows indicate the CpG sites analysed in the methylation-sensitive PCR assay. Filled boxes represent exons. The genomic positions refer to the 2009 (GRCh37/hg19) genome assembly. (b) SOCS3 CpG methylation percentage in T2DM patients and controls. Data are mean ± SD. *p ≤ 0.05, **p ≤ 0.01 (Student t-test). (c) Box-whisker plots of SOCS3 mRNA levels in T2DM patients and controls. Boxes show the median, the 25th and the 75th percentiles. Whiskers show the minimum and the maximum data point. **p ≤ 0.01 (Student t-test). N = 48 CT, 61 T2DM