Figure 2.

Effects of gossypol exposure on neural stem cell (NSC) proliferation in the dentate gyrus (DG) and newborn granule cells (GCs). (a) Representative images of BrdU (green) and Prox1 (red) labeling of newly generated cells in the DG at 2 hours after BrdU injection at postnatal day (P) 8. Brain sections were counterstained with PI (blue). (b) Higher magnification of newly generated cells (white arrows). (c) Schematic diagram of the DG. The DG could be roughly divided into 3 layers: the hilus, the molecular layer (Mol), and the GC layer (GCL), including the subgranular zone (SGZ). (d–g) Quantification of the number of BrdU-labeled newly generated cells in the DG (d) and GCL (e), BrdU⁺ Prox1⁺ cells in the hilus (f) and GCL (g). There were significant differences in the number of BrdU cells and the ratio of BrdU⁺ Prox1⁺ cells to the total BrdU⁺ cells among the 3 groups of offspring. Data represent the mean ± SEM (n = 10 per group, 10 sections from 5 offspring in each group). Scale bars = 100 μm. *P < .05 and **P < .01 compared with the controls.