Fig. 3.

Robustness of the rrnB P1 ATP reporter in different E. coli strains. a Dynamics of cellular GFP (GFP/OD) of four E. coli strains (NEB 10-beta, BW25113, JM109DE3, and BL21DE3) with HC-M ATP reporter in rich medium with 6 mM glucose. b Corresponding bacterial growth among the four strains in the same medium. Bacterial strains were grown in black 96-well plates and GFP (ex485/em528) and OD600 were measured by a microplate reader. Cellular GFP (GFP/OD) signals of each strain were normalized to their respective peak GFP/OD values (100%) for comparison among different strains. Each data point represents the mean of at least three independent replicates with a standard deviation < 13% of the mean