Table 1.
Lag phase | Exponential phase | Stationary phase | ||||
---|---|---|---|---|---|---|
Luc | Reporter | Luc | Reporter | Luc | Reporter | |
Averaged ATP (mM) | 0.86 ± 0.16 | 0.72 ± 0.12 | 3.50 ± 0.45 | 3.32 ± 0.28 | 0.58 ± 0.08 | 0.68 ± 0.07 |
ATP consumption rate (million ATP/cell/s) | 0.82 ± 0.07 | 0.77 ± 0.11 | 6.38 ± 0.06 | 6.38 ± 0.06 | 0.23 ± 0.06 | 0.23 ± 0.06 |
Power consumption (pW)a | 0.073 ± 0.006 | 0.069 ± 0.01 | 0.57 ± 0.01 | 0.57 ± 0.005 | 0.021 ± 0.005 | 0.021 ± 0.005 |
ATP turnover time (s) | 0.64 ± 0.12 | 0.56 ± 0.09 | 0.33 ± 0.04 | 0.31 ± 0.03 | 1.5 ± 0.21 | 1.8 ± 0.18 |
a: power consumption was estimated from 54 kJ/mole ATP [53, 54]. All values were estimated from three independent biological experiments evaluated by the kinetic model. Luc indicates the calculated ATP data based on the luciferase assay while Reporter indicates ATP data based on the measurements from our GFP reporter