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. Author manuscript; available in PMC: 2021 May 18.
Published in final edited form as: Methods Enzymol. 2018 Oct 22;611:101–136. doi: 10.1016/bs.mie.2018.09.011

Fig. 2.

Fig. 2

Resonance overlap problem for IDPs. (A) 1H–15N HSQC of MdmX residues 23–210, a protein with both an ordered domain and disordered domain (Fig. 1). Inset shows crowding in the central region of the spectrum due resonance overlap from disordered residues. Panel (B) shows 1H–13C planes of correlated i and i – 1 spin systems from the HNCACB spectrum of four residues and the connectivity of the resonances. Blue resonances correspond to alpha carbons (>45 PPM) and red resonances correspond to beta carbons (<45 PPM). Each panel shows the correlated spin systems for residue i and i – 1. Arrows connect i – 1 resonances from one spin system with the i resonances of the previous spin system.