VSDI analysis and pharmacological validation of evoked neuronal activity propagations through the LA-to-CeA network. A, VSDI filmstrip depicting the spread of neuronal activity triggered by an electrical stimulation pulse (Stim) delivered to the most dorsal part of the LA (for color bar, see B). B, ROI-based extraction of LA and CeA FDSs. C, Coapplication of APV and NBQX to slices (n = 3) strongly diminished the LA and CeA FDSs. Residual FDS components were fully abolished by TTX. D, BIM enhanced the LA and CeA FDSs (n = 6). A–D, All experiments were performed in slices from NAB mice.