Fig. 2.

Increased CB₂ expression is an early event in tauopathies. Analysis of 7 months old hTAU^P301S transgenic mice. a Neuronal location of the CB₂ receptor in the hippocampus (CA2) of 7 months old hTAU^P301S transgenic mice. Immunofluorescence with anti-mTAU (wild type mice) or anti-hTAU (transgenic mice) (red), anti-CB₂ (green), and nuclear staining with DAPI (blue). b Quantification of Cnr2 and Cnr1 mRNA levels, n = 4 samples ± SEM. The data has been processed with Student's t-test analysis to determine the significance of the changes. The asterisks represent the difference in significance * p < 0.05. c Neuronal location of the CB₂ receptor in the hippocampus (CA2) in the AAV-hTAU^P301L mouse model. Immunofluorescence with anti-hTAU (red), anti-CB₂ (green), and nuclear staining with DAPI (blue). d Analysis of wild type mice injected into the ipsilateral hippocampus with the AAV-hTAU^P301L vector for 3 weeks. Quantification of Cnr2 and Cnr1 mRNA levels, n = 4–6 samples ± SEM. The data has been processed with Student's t-test analysis to determine the significance of the changes. The asterisks represent the difference in significance **** p < 0.0001